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yjbH [2017-05-24 16:06:18]
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yjbH [2017-05-24 16:06:18]

adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents
locus
BSU11550
pI
5.21
mw
31.33 kDa
protein length
275 aa Sequence Blast
gene length
825 bp Sequence Blast
function
stimulation of Spx degradation
product
adaptor protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

    • Gene

    Coordinates
    1,233,614 → 1,234,513

    Phenotypes of a mutant

  • increased thermotolerance due to increased stabiliy of [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] and thus increased expression of ''[gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]'' [Pubmed|24417481]

    • The protein

    Catalyzed reaction/ biological activity

  • adaptor protein for [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|ClpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]-catalyzed [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] degradation [Pubmed|19074380]

    • Protein family

  • UPF0413 family (according to Swiss-Prot)

    • [SW|Cofactors]

  • contains Zn atoms (coordinated by the N-terminal His-rich region) [Pubmed|19074380]

    • Effectors of protein activity

  • [protein|87124945A1CBF7990856FBEB9EAD25096DAC0868|YjbH] aggregates under stress conditions, resulting in the inability to bind to [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx], and therefore in stabilization of [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] [Pubmed|25353645]
  • Zn atom is released upon treatment with strong oxidants [Pubmed|19074380]
  • interaction with [protein|5EC57A51EF2DC48070615653B7A3C1007CFEE01D|YirB] inhibits the formation of a complex with [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] [Pubmed|21378193]

    • [SW|Localization]

  • cytosolic protein

    • Expression and Regulation

    Operons

    genes
    [gene|948BD57CC45915C7CDDB8EF168287533FC514B7C|yjbI]-[gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|yjbH]
    description
    [Pubmed|17293416]

    regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|29271514], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|29271514]

    • additional information

  • A [protein|search|ncRNA] is predicted between [gene|138DC46C976BAA18893B70DF040BA1BB2FA0A77C|yizD] and [gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|yjbH] [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • BKE11550 (''[gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|yjbH]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Peter Zuber], Oregon Health and Science University, USA
  • [http://www.ogi.edu/people/dsp_person.cfm?person_id=411D6801-2A56-D16D-58A06B4480EDB9C7 Homepage]
  • [SW|Claes von Wachenfeldt], Lund University, Sweden [http://aron.ldc.lu.se/kundwebb/cellorg/mibiol/research/wachen/index.htm Homepage]

    • References

    Reviews

  • 23375660,23479438,19609260

    • Original Publications

  • 17293416,19074380,17908206,20525796,21378193,21947404,24417481,24942655,25353645,27191337